Human Neurofilament H ELISA Kit (200 kDa Neurofilament protein)
$676.00
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 141 pg/ml
- Range: 0.94 ng/ml – 60 ng/ml
- Sample type: Cell Lysate, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
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Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 6% Inter-assay Sample n Mean SD CV% Extract 3 2.1% -
Sample type
Tissue Extracts, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
141 pg/ml -
Range
0.94 ng/ml – 60 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Tissue Extracts 120 115% – 127% Cell Lysate 86 82% – 90% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Neurofilament H ELISA kit (ab272199) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Neurofilament H protein in human cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Neurofilament H with 1.41 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Neurofilament H, also known as 200 kDa Neurofilament Protein, together with Neurofilament L and M are three intermediate filament proteins of neurofilaments. Neurofilament H is involved in the maintenance of neuronal caliber and may play a role in intracellular transport. Neurofilament H is used as a neuronal damage biomarker and mutations to the gene have been associated with susceptibility to amyotrophic lateral sclerosis. The human Neurofilament H amino acids sequence is 75% identical to mouse and rat Neurofilament H sequence.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
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