,

Human IL-6 ELISA Kit

$711.00

Key features and details

  • Sensitivity: 2 pg/ml
  • Range: 6.25 pg/ml – 200 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human
Categories: ,

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Pooled sampl 15 4.2%
    Inter-assay
    Sample n Mean SD CV%
    Sample 1 18 200pg/ml 16 8.1%
    Sample 2 18 77pg/ml 8 10.1%
    Sample 3 18 63pg/ml 6 8.6%
    Sample 4 18 103pg/ml 6 6%
    Sample 5 18 53pg/ml 3 5.5%

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    < 2 pg/ml

  • Range

    6.25 pg/ml – 200 pg/ml

  • Recovery

    96 %

  • Assay time

    1h 45m

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam’s IL-6 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-6 in Human serum, plasma, buffered solutions or supernatants.

    A monoclonal antibody specific for IL-6 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-6 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-6 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-6 present in the samples.

    This kit will recognize both endogenous and recombinant Human IL-6.

    Get higher sensitivity in only 90 minutes with Human IL-6 ELISA Kit (ab178013) from our SimpleStep ELISA® range.

  • Platform

    Microplate

Related products

  • Uncategorized

    Cleaved Tau ELISA Kit (Human Asp738/Mouse Asp713)

     $660.00 Add to cart

     

    • Key features and details

      • One-wash 90 minute protocol
      • Sensitivity: 0.075 ng/ml
      • Range: 0.313 ng/ml – 10 ng/ml
      • Sample type: Cell culture media, Cell culture supernatant, Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
      • Detection method: Colorimetric
      • Assay type: Sandwich (quantitative)
      • Reacts with: Mouse, Human
  • Placeholder
    Analytical Testing

    Vomitoxin/Deoxynivalenol (DON) – ELISA 1 to 3 samples

     $550.00 Add to cart
  • Uncategorized

    Human Tau ELISA Kit

     $699.00 Add to cart

    Key features and details

    • One-wash 90 minute protocol
    • Sensitivity: 16 pg/ml
    • Range: 40.63 pg/ml – 2600 pg/ml
    • Sample type: Cell culture extracts, Cell culture media, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human
  • Uncategorized

    S100B ELISA Kit

     $754.00 Add to cart

    Key features and details

    • One-wash 90 minute protocol
    • Sensitivity: 139 pg/ml
    • Range: 0.31 ng/ml – 20 ng/ml
    • Sample type: Tissue Extracts
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Mouse, Rat, Human
Shopping Cart