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Human IL-6 ELISA Kit

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Pooled sampl 15 4.2%
    Inter-assay
    Sample n Mean SD CV%
    Sample 1 18 200pg/ml 16 8.1%
    Sample 2 18 77pg/ml 8 10.1%
    Sample 3 18 63pg/ml 6 8.6%
    Sample 4 18 103pg/ml 6 6%
    Sample 5 18 53pg/ml 3 5.5%

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    < 2 pg/ml

  • Range

    6.25 pg/ml - 200 pg/ml

  • Recovery

    96 %

  • Assay time

    1h 45m

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam’s IL-6 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-6 in Human serum, plasma, buffered solutions or supernatants. A monoclonal antibody specific for IL-6 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-6 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-6 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-6 present in the samples. This kit will recognize both endogenous and recombinant Human IL-6. Get higher sensitivity in only 90 minutes with Human IL-6 ELISA Kit (ab178013) from our SimpleStep ELISA® range.

  • Platform

    Microplate
$711.00
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Min: 1
Step: 1
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Human Tau
Human Tau levels are determined using Enzyme-Linked Immunosorbent Assay (ELISA) technology.
3
$737.00
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Min: 1
Step: 1
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Iodine value
Iodine value of the oil is determined by the American Oil Chemists’ Society (AOCS) recommended practice Cd 1c-85 (re-approved 2009). Iodine value is calculated using the fatty acid composition of the oil following the International Organization for Standardization (ISO) Methods 12966-1:2014 and 12966-2:2011.
2
$630.00
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Min: 1
Step: 1
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Mycotoxin confirmatory analysis
The mycotoxin content of the sample, initially obtained by enzyme-linked immunosorbent assay (ELISA) technology or ultra-high performance liquid chromatography (UPLC) with fluorescence detection, is assessed using liquid chromatography-tandem mass spectrometry.
7
$510.00
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Min: 1
Step: 1
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Ochratoxin A (OTA) – 2 to 3 results
OTA content of the sample is tested by Ultra-High Performance Liquid Chromatography (UPLC) with fluorescence detection.
7
$1,147.00
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Min: 1
Step: 1
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Ochratoxin A (OTA) – 4 or more results
OTA content of the sample is tested by Ultra-High Performance Liquid Chromatography (UPLC) with fluorescence detection.
7
$796.00
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Min: 1
Step: 1
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Ochratoxin A (OTA) – single result
OTA content of the sample is tested by Ultra-High Performance Liquid Chromatography (UPLC) with fluorescence detection.
7
$2,096.00
Max:
Min: 1
Step: 1
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Protein CNA
Protein content of the sample is determined by Combustion Nitrogen Analysis (CNA).
3
$322.00
Max:
Min: 1
Step: 1
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Protein NIR
Crude protein content of the sample is determined by method Near-Infrared Reflectance (NIR). With appropriate grain samples, American Oil Chemists’ Society (AOCS) Official Method Ba4e-93 (revised 2011) is used to develop the calibration.
2
$365.00
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Min: 1
Step: 1
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Protein NIT
Protein content of the sample is determined by Near-Infrared Transmittance (NIT) FOSS Infratec 1241 – limited to existing installed calibrations.
2
$107.00
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Min: 1
Step: 1
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