Detection method

Colorimetric
Precision

Intra-assay

Sample n Mean SD CV%

Overall 60 <=9%

Inter-assay

Sample n Mean SD CV%

Overall 30 <=9.8%
Sample type

Urine, Serum, Hep Plasma, Cit plasma
Assay type

Competitive
Sensitivity

= 2.44 ng/ml
Range

10 ng/ml – 500 ng/ml
Recovery

103.56 %
Assay duration

Multiple steps standard assay
Species reactivity

Reacts with: Human
Product overview

Abcam’s Cortisol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cortisol in serum and plasma.

A 96-well plate has been precoated with anti-Cortisol IgG. Samples and the Cortisol-HRP conjugate are added to the wells, where any Cortisol in the sample competes with the added Cortisol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Cortisol in the sample and the intensity is measured at 450 nm.

Cortisol ELISA Kit

Contact us