Detection method

Colorimetric
Sample type

Cell culture supernatant, Serum, Plasma
Assay type

Sandwich (quantitative)
Sensitivity

< 8 pg/ml
Range

8.19 pg/ml – 2000 pg/ml
Recovery

> 81 %

Sample specific recovery

Sample type Average % Range

Cell culture supernatant 82.77 70% – 96%

Serum 91.93 76% – 106%

Plasma 81.32 69% – 97%
Assay duration

Multiple steps standard assay
Species reactivity

Reacts with: Human
Product overview

Abcam’s ErbB2 (Epidermal Factor Growth Factor Receptor 2) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ErbB2 in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for human ErbB2 coated on a 96-well plate. Standards and samples are pipetted into the wells and ErbB2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human ErbB2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ErbB2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Human ErbB2 / HER2 ELISA Kit

Contact us